pathological diagnosis

Spefor Animal Pathology Diagnosis Center provides professional animal pathology technology services. Provides high-quality tissue image production and pathological interpretation for animal inspection and organ specimens through nationally certified veterinary pathologists and technical specialists in animal pathology. The platform is staffed by a senior expert team with over 40 years of clinical pathology experience, providing technical services and support for histomorphological research and drug safety evaluation.

Team and equipment

Dr. Li Li, graduated from the pathology department of Changchun University of Agriculture and Animal Husbandry of the Chinese People's Liberation Army in 1995, has been engaged in pathology research for nearly 40 years. Currently serves as the chief technical support scientist of Spafford (Beijing) Biotechnology Co., Ltd. Professor Li has rich experience in experimental animal and pathology research and teaching, mainly responsible for technical support, pathology services, and animal modeling related research work.

Dr. Li served as the director of the Experimental Animal Center of the Eighth Medical Center of the PLA General Hospital (formerly the PLA No. 309 Hospital), and participated in nearly 100 national and military scientific research projects. He has served as the director and professor of the Pathology Teaching and Research Department of the Beijing Military Medical College and a specially appointed professor at Beijing Institute of Technology. He also serves as a member of the Military Medical Experimental Animal Professional Committee and the Military Veterinary Health Professional Committee. He has participated in the national "Twelfth Five-Year Plan" and "Thirteenth Five-Year Plan" major infectious disease projects, completed more than 10 national natural science fund projects, participated in more than 20 military scientific research projects, obtained 8 national invention patents, published more than 40 academic papers, and won the first prize of the Beijing experimental animal industry and the third prize of the scientific and technological progress of the Chinese People's Liberation Army.

Paraffin section:

The most widely used method in routine histological preparation techniques. Paraffin sections are not only used to observe the morphological structure of normal cell tissue, but also the main method for studying, observing and judging the morphological changes of cell tissue in disciplines such as pathology and forensic medicine. They have also been widely used in research in many other fields. Frozen section:

A method of quickly cooling tissue to a certain hardness under low temperature conditions and then slicing it. Because of its faster and simpler production process than paraffin sections, it is mostly used for rapid pathological diagnosis in surgery.

Fresh brain tissue vibratome sectioning:

During vibratome sectioning, the tissue is thickly sliced by a blade that vibrates at high frequency, with a slice thickness of 30-999um. The tissue specimen does not need to be frozen or ded during sectioning, reducing damage from ice crystals to the tissue and maintaining sample activity and cell morphology. Vibratome tissue sectioning can compensate for the effects of thin sectioning on the abundance of certain components within the tissue, such as nerve fibers and blood vessels, caused by paraffin or frozen sectioning.

Staining and imaging

HE staining: also known as hematoxylin-eosin staining, is one of the commonly used staining methods in paraffin section technology. Hematoxylin is alkaline and mainly stains the chromatin in the cell nucleus and nucleic acid in the cytoplasm purple-blue, while eosin is acidic and mainly stains the components in the cytoplasm and extracellular matrix red. HE staining is the most basic and widely used technical method in histology, embryology, pathology teaching and research.

Safranin-O/fast green staining: Alkaline-loving cartilage combines with the alkaline dye safranin-O to appear red, while acid-loving bone combines with the acid dye fast green to appear green or blue, which contrasts sharply with the red cartilage, thus distinguishing between cartilage and bone tissue.

Masson staining: is one of the staining methods used to detect collagen fibers in animal tissues. It can stain collagen fibers blue, muscle fibers and red blood cells red, and can be used to identify collagen fibers and muscle fibers, and display the content and degree of fibrosis of various tissue collagen fibers.

PAS staining: also known as periodic acid-Schiff staining, glycogen staining. Generally used to display glycogen and other polysaccharides. Periodic acid can oxidize the ethylene glycol groups of polysaccharides in cells into aldehydes, which then combine with Schiff's reagent to locate red on the cytoplasm. It can observe changes in glycogen in the liver, muscle tissue, and acidic mucus substances in cup-shaped cells such as the gastrointestinal tract and bronchi.

Oil Red O staining: Oil Red O is a lipophilic dye that can highly dissolve in fat and specifically stain neutral lipids such as triglycerides in tissues.

Immunohistochemistry: A technique that uses labeled specific antibodies or antigens to detect antigens or antibodies in tissues and cells for localization, qualitative and relative quantitative research. Immunohistochemistry can be applied to pathological diagnosis and scientific research of malignant tumors; it can be combined with morphological diagnosis of histochemical staining methods to improve the accuracy of differential diagnosis of difficult tumors, and can provide guidance and selection of clinical treatment plans for further pathological classification of tumors.

Fluorescence imaging: Immunofluorescence technology combines fluorescence labeling technology and immunological methods to locate, trace, qualitatively and relatively quantitatively antigen or antibody substances in tissues or cells through antigen-antibody interactions. Immunofluorescence technology has strong specificity, high sensitivity, fast reaction speed, and clear and definite results.

Tissue pathological analysis:

◎ Reading and diagnosis of slides; ◎ Pathological scoring; ◎ Analysis of medical records; ◎ Special pathological research.